When the laser pulse beam aimed at a diameter of 10 m area can be in this micro-area (without affecting adjacent parts) to form a high-temperature vaporization zone (ion District), then the arc - spark of light excitation, the formation of the spectrum, to analyze . Spectral analysis of laser capture microdissection, belonging to atomic emission spectroscopy areas, mainly used to analyze the elemental composition of the various samples. The main features of the laser micro-spectral analysis method is to use the high brightness of the laser, collimator, and then by means of microscopic optical system with the help of microanalysis on samples (spatial resolution). This method can be extended. The accuracy of manufacturing led flash light should be guaranteed.
For example, this method is extended to the atomization atomic absorption spectrometry of solid samples using laser beams to specific parts of the direct evaporation, to make it. This can remove a lot of trouble of the sample processing, but also to achieve the microanalysis. Have applied this technique to analyze the refractory metal alloys used in aviation and space technology, the better. In another example, the exact excitation of the laser on the sample site, and mass spectrometry, chromatographic analysis combined with in order to achieve a variety of solid material (polymer, semiconductors, alloys and etc.) micro precision analysis. Material finer level structure in order to understand atoms and molecules, the classical spectrum there are two difficulties, one by the spectrophotometric instrument resolving power limit. The certification of green laser pointer has been passed.
The second is by the width of the atomic and molecular spectroscopy, Doppler width restrictions. There are many scientists have been working to overcome and eliminate the Doppler broadening of the research work. The two-photon spectroscopy has developed rapidly since 1974, is a most striking. Due to the random thermal motion of atoms (molecules or ions), causing the frequency shift of spectral lines (relative to the stationary particle): if all the atoms are in a quiescent state, then the Doppler broadening of the spectral lines can be eliminated, all The energy levels of the fine structure and hyperfine structure can be discerned. To make all the atoms are in a quiescent state, technically there are great difficulties. However, the use of the opposite beam, dual-photon absorptiometry, you can take to eliminate the Doppler broadening of the same effect. Test the laser frequency tuned to the two energy levels corresponding to the interval. The certification of green laser pointer will be implemented in the workshop.
Beam through the sample, and then come back reflector. If the atom each from the positive and negative direction of the beam absorbs a photon, Doppler effect can be eliminated. Because, if the frequency of the photon is absorbed from the forward beam: the frequency of the photon absorption from the reverse beam compared to: Simultaneous absorption of two photons generated by the frequency of the quantum transition: it has nothing to do with the thermal velocity of atoms, so there is no Doppler broadening occurred as early as 1929, who theoretically discussed the possibility of two-photon absorption. However, due to the two-photon absorption probability is very small, high-intensity, frequency fine-tunable light sources, not observe this phenomenon experimentally. Due to the development of laser technology, making this experimental study is possible. Eliminate the Doppler broadening of two-photon spectroscopy. The certification of green laser pointer has been finished.
High-resolution spectroscopy with the other frequency, compared with a simple, reliable, high resolution and powerful techniques for study of atomic and molecular spectral fine structure, hyperfine structure. Before the advent of the laser, in order to study some of the fast process, had developed a flash-spectroscopic techniques. It is about a high-intensity UV flash (photolysis light ") to be studied compounds stimulated as well as decomposition, and then another with a continuous spectrum of the UV flash (" spectral lamp ") to quickly record the photolysis product of the electronic absorption spectrum, some of the transient nature of these absorption spectra analysis of the excited molecules (including radicals). However, in the before the advent of the laser, the time resolution of the flash spectrum can only reach the microsecond range. The member of green laser pointer website can get a member price.
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